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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the mechanisms of antigenic variation in Trypanosoma brucei, the causative agent of African trypanosomiasis. The parasite evades the host immune response by periodically changing its surface coat protein. This process primarily involves:

A) RNA interference-mediated silencing of expressed VSG genes followed by activation of a new VSG gene.
B) Segmental gene conversion events involving a large repertoire of silent VSG genes.
C) High-frequency point mutations in the gene encoding the variant surface glycoprotein (VSG).
D) Recombination between different VSG gene loci on minichromosomes.

2. A clinical microbiology laboratory is investigating a potential outbreak of Legionella pneumophila associated with a hospital's water system. The MOST appropriate environmental samples to collect for culture and comparison to clinical isolates would be:

A) Air samples from patient rooms and common areas.
B) Water samples from showers, faucets, and cooling towers.
C) Surface swabs from frequently touched objects.
D) Sputum samples from asymptomatic healthcare workers.

3. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV-1 p24 antigen assay only
B) HIV-1/HIV-2 antibody differentiation immunoassay
C) HIV RNA nucleic acid test (NAT)
D) HIV-1/HIV-2 antigen/antibody combination immunoassay

4. A clinical microbiology laboratory is monitoring the emergence of resistance to colistin in Gram-negative bacteria. Resistance to colistin is often mediated by modifications of the bacterial lipopolysaccharide (LPS).
The genetic basis for acquired colistin resistance most commonly involves:

A) Alterations in the outer membrane porin proteins, reducing colistin uptake.
B) Acquisition of genes encoding efflux pumps that specifically target colistin.
C) Production of enzymes that directly inactivate the colistin molecule.
D) Mutations in genes encoding enzymes that modify the lipid A component of LPS.

5. Besides traditional Giemsa-stained thick and thin blood smears, which diagnostic approach is commonly employed as a rapid diagnostic test (RDT) for malaria detection, particularly in resource-limited settings?

A) Serological detection of anti-Plasmodium IgG antibodies
B) Immunochromatographic assays detecting Plasmodium-specific antigens (e.g., HRP2, pLDH)
C) Quantitative Buffy Coat (QBC) analysis
D) PCR amplification of Plasmodium 18S rRNA gene directly from saliva

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